SR7500DC SPR System

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SR7500DC Dual Channel Surface Plasmon Resonance System

Dual channel SPR system with unparalleled sensitivity in protein interaction analysis, pushing detection limits with extremely low noise (0.05μRIU) and drift (0.01μRIU/min).

Reichert's SR7500DC pushes the limits of detection and sensitivity in label-free interaction analysis.

The SR7500DC Dual Channel System is the next generation of surface plasmon resonance (SPR) technology with incomparable sensitivity and high-quality binding data for interaction analysis - all in a flexible component-based platform. The key to the system is the advanced SR7500 Dual Channel SPR Spectrometer instrument.

The SR7500DC label-free system generates data on critical interactions between proteins, nucleic acids, lipids, carbohydrates, small molecules, whole cells, bacteria, viruses, and polymers.

The SR7500DC System is used in pharmaceuticals, drug discovery, antibody screening, protein structure and function, gene regulation, and systems biology.

Exceptional sensitivity in the SR7500DC System provides real-time data and is ideal for:

High quality kinetic analysis
Affinity measurements ranging from 1mM to 1pM
Precise determination of thermodynamic parameters
Accurate concentration analysis

Details of the SR7500DC System

Exceptional sensitivity starts with extreme low noise (0.05 µRIU) and extreme low drift (0.01 µRIU)

Binding of 4-carboxybenzenesulfonamide to immobilized carbonic anhydrase II

High sample capacity (up to 768 samples)
Temperature control from 10° C below ambient to 70° C
Fast data sampling rates (up to 10 Hz)
Broad refractive index range (1.32 to 1.52)
Monitor binding of low molecular weight compounds (<95Da)
Identify potential drug targets and therapeutics
Antibody characterization
Uses two RL1210 Perkin Elmer Photodiode 1024 pixel arrays featuring 100,000:1 dynamic range and 0.4 picoAmp dark current.

Modular component-based design

Ultimate flexibility for cost-effective biomolecular interaction analysis
Minimal maintenance requirements with off-the-shelf fluidics
Low life cycle costs
All components are purchased separately or as a system providing for easy upgrades.
User-maintainable with minimal maintenance required, eliminating the need for yearly service contracts.

Sophisticated, intuitive software

Autolink is integrated, sophisticated software
21 CFR part 11 controls
Altera FPGA with a virtual soft-core 32 bit processor
Processor is field programmable for future upgrades

Flow Cells	Sensor Chips	Fluidics Kit
Reichert offers a standard flow cell with each system. This flow cell is used to perform molecular interaction studies and has low channel volume resulting in extremely fast solution exchange dynamics. Reichert also offers specialized cells that provide the ability to couple SPR measurements with other valuable analytical techniques opening up new avenues for investigation.	Reichert sensor chips are offered at affordable prices so researchers can explore more interactions without higher running costs. Choose from plain gold sensor chips, carboxymethyl dextran chips, planar polyethylene glycol/carboxyl chips, nickel nitrilotriacetic acid chip, hydrophobic planar alkyl chip, streptavidin/neutrAvidin chip, and protein A chip.	The Reichert SPR systems are designed to use low-cost, off-the-shelf-HPLC fittings and tubing, allowing for quick and easy changeover. The kits provide all the connectors and tubing needed to properly plumb the system, including spares.

SR7500DC Specifications

Technical Information

Transducer Principle – Kretschmann prism, multiple angles from fixed light input
Measurement Channels – Two (either parallel or series fluid connection)
Sample Loading – Autosampler or semi-automatic injector, standard HLPC tubing and connectors, external syringe pump
Sample Capacity – Any combination of up to 2 trays can be used. Choose: 12 (10 mL) or 48 (2 mL) Vials; or 96-well (high or low) or 384-well Plates
Sample Storage – 4°C or ambient temperature
Flow Cell Volume per Channel – 0.18 µL
Flow Cell Surface Area per Channel – 4.5 mm² (reference value)
Aspect Ratio – >25 (width/height)
Fluid Contact Materials – Teflon™, Acetal Copolymer, PEEK™, Kalrez™, ETFE (Tefzal™)
Sample Volume – 1 to 5000 µL (depends on installed loop volume)
System Fluid Volume – (typically) 28 µL (0.01" I.D. tubing) or 7.5 µL (0.005" I.D. tubing)
Temperature Range – 10°C below ambient to 70°C

Measurement Sensitivity

Refractive Index Resolution – < 10-7 RIU (< 0.1 µRIU)
Refractive Index Range – 1.33 to 1.40 (@780nm)
Analyte Concentration Range – 1 mM to 1 pM
Minimum Molecular Weight Detection – < 100 Daltons
Baseline Noise – 0.1 µRIU peak-to-peak, 0.05 µRIU RMS, @ 25 mL/min
Baseline Drift – < 0.01 µRIU/min

Typical Kinetic and Equilibrium Constant Ranges

Association Rate Constant – 103 to 107 M-1s-1
Dissociation Rate Constant – 10-1 to 10-5 s-1
Equilibrium Dissociation Constant – 0.1 mM to 1 pM

Electrical	Regulatory	Product Safety
AC Power Supply, standard international voltage range w/universal adapter from 100 to 240 V & 50 to 60 Hz	Compliance with the applicable sections of the European EMC Directive and IEC safety requirements for laboratory electrical equipment for measurement and control	Compliance with IEC 61010-1 (Low Voltage Directive) under a Category classification EMC and Safety: CE mark certification (Class A, Type II)